A Digital Microfluidic Platform for Automated Immunoassays Optimized Using “design of Experiments” (doe) Methods

We introduce an automated digital microfluidic platform capable of performing immunoassays from sample-toanalysis with minimal manual intervention. This platform features a 90 Pogo pin interface, an integrated photomultiplier tube, and an adjustable magnet. The new platform was used to implement a design of experiments optimization for immunoassays, resulting in an optimized protocol that reduced detection limit and sample incubation time by up to 5-fold and 2-fold, respectively, relative to previous work. We propose that this new platform paves the way for a benchtop tool that is useful for implementing immunoassays in near-patient settings around the world.